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Development Of Novel Droplet-based Microfluidic Strategies

 
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MessagePosté le: Dim 18 Juin - 06:59 (2017)    Sujet du message: Development Of Novel Droplet-based Microfluidic Strategies Répondre en citant



The aim of this work is to establish novel strategies for the highly sensitive screening of cancer biomarkers in biological samples.To achieve this goal, we developed droplet-based microfluidic dPCR technique. Using a limiting dilution, individual DNA molecules are encapsulated within monodisperse droplets of a water-in-oil emulsion created with a microfluidic device. Fluorescent TaqMan probes targeting the screened cancer biomarkers allow the detection of mutations. We focused on the mutations in the human KRAS gene for the development of our test. This method is also transposable in a multiplexed format for the parallel detection of multiple mutations in clinical samples.The developed technique allowed the precise quantification of a mutated KRAS gene in the presence of a 200,000-fold excess of un-mutated KRAS genes and enabled the determination of mutant allelic specific imbalance (MASI) in several cancer cell-lines.


Details:
rank:
price: $69.40
bound: 212 pages
publisher: Éditions universitaires européennes (March 24, 2017)
lang: English
asin:
isbn: 3639623177, 978-3639623178,
weight: 12.8 ounces (
filesize:




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MessagePosté le: Dim 18 Juin - 06:59 (2017)    Sujet du message: Publicité

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